Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio

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Effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (Bubalus bubalis) spermatozoa

AIM The aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (CLC) treated buffalo spermatozoa. MATERIALS AND METHODS Semen samples with mass motility of 3+ and greater, collected from Murrah buffalo bulls were utilized. Immediately after collection, four equal groups of semen sample were made. Group I was kept as control and diluted...

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Mensuration of spermatozoa from different levels of the reproductive tract of the buffalo - bull ( Bubalus bubalis )

The dimensions of spermatozoa from different levels of the reproductive tract were measured in 6 buffalo-bulls castrated under local anesthesia ; the same measurements were taken in the semen of 6 breeding buffalo-bulls. During transit from caput to cauda epididymis the sperm showed a significant increase in the length of head and main piece. However, a significant decrease was observed in midd...

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Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa.

Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the maj...

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INFECTIOUS CAUSES OF INFERTILITY IN BUFFALO BULL (Bubalus bubalis)

The artifi cial insemination (AI) technique plays an important role in improvement of conception rate, prevention of sexually transmitted diseases and transmission of genetic material to the next generation. The breeding soundness evaluation (BSE) plays an important role in selection of male buffalo for the said purposes. This evaluation is an effective, inexpensive and easy method for selectio...

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Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa

OBJECTIVE The aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or Bioxcell®) on cryopreservation of buffalo semen. MATERIALS AND METHODS In this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in Bioxcell® and tris-citric ...

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ژورنال

عنوان ژورنال: Veterinary World

سال: 2014

ISSN: 0972-8988,2231-0916

DOI: 10.14202/vetworld.2014.702-706